Preparation of Agarose with Low Net Negative Charge Density Using an Inexpensive Anion Exchanger
- 1 December 1986
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 16 (4) , 309-319
- https://doi.org/10.1080/00327488608068751
Abstract
A method of preparing agarose of low net negative charge density by the use of Chromium tanned gelatin is described. Using commercial agarose (agarose c) as standard of comparison the product has a sulphate content approximately 0.1 that of agarose c and its pyruvate content was reduced to zero as shown by NMR in DMSO. The product was still able to adsorb and desorb serum glycoproteins similar to agarose c indicating that charge on the agarose c gel particles played a minor role if any on the binding of the serum glycoproteins.This publication has 3 references indexed in Scilit:
- Binding and Elution of Serum Glycoproteins from AgarosePreparative Biochemistry, 1984
- Preparation of agarose by fractionation from the spectrum of polysaccharides in agarAnalytical Biochemistry, 1971
- A method of preparing agaroseBiochimica et Biophysica Acta (BBA) - General Subjects, 1964