A rapid enzymic procedure for the determination of picomole amounts of UDP-glucuronic acid
- 1 August 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 189 (2) , 369-372
- https://doi.org/10.1042/bj1890369
Abstract
A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalyzed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 .mu.g of cellular protein.This publication has 7 references indexed in Scilit:
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