Ecdysone Oxidase: Reaction and Specificity
Open Access
- 1 September 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 89 (2) , 453-460
- https://doi.org/10.1111/j.1432-1033.1978.tb12548.x
Abstract
Ecdysone oxidase oxidizes 3-hydroxysteroids of the ecdysteroid type irreversibly to 3-dehydro derivatives. The hydrogen of the steroid is transferred by the enzyme to oxygen which is reduced to hydrogen peroxide. Ecdysone oxidase is relatively specific for ecdysone and ecdysterone. Apparent Michaelis constants for these two physiological substrates are 98 and 31 μM, respectively. Cholesterol oxidase and 3α-hydroxysteroid and 3β-hydroxysteroid dehydrogenases which catalyze similar reactions are unable to oxidize ecdysteroids.This publication has 21 references indexed in Scilit:
- Evidence for an α-ecdysone cytochrome P-450 mixed function oxidase in insect fat body mitochondriaNature, 1977
- Enzymatic and Chemical Synthesis of 3-Dehydroecdysterone, a Metabolite of the Moulting Hormone of InsectsHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- Ecdysone 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)Cellular and Molecular Life Sciences, 1976
- The
- Recent Developments in Insect Steroid MetabolismAnnual Review of Entomology, 1975
- Ecdysone Oxidase, an Enzyme from the BlowflyCalliphora erythrocephala(Meigen)Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1975
- Biosynthesis of ecdysones in isolated prothoracic glands and oenocytes of Tenebrio molitor in vitroJournal of Insect Physiology, 1974
- The secretion and metabolism of α-ecdysone by cockroach (Leucophaeamaderae) tissues invitroLife Sciences, 1974
- Biosynthesis of α-Ecdysone by Prothoracic Glands in vitroScience, 1974
- Formation of Lichen Substances by Mycobionts of Lichens. Isolation of (+) Usnic Acid and Salazinic Acid from Mycobionts of Ramalina spp.CHEMICAL & PHARMACEUTICAL BULLETIN, 1969