Megapixel digital PCR
Top Cited Papers
- 3 July 2011
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 8 (8) , 649-651
- https://doi.org/10.1038/nmeth.1640
Abstract
This digital PCR device arrays samples into one million small-volume reactors, achieving a dynamic range of 107, measurement precision better than 1% and the ability to detect single-nucleotide variants present at less than 1:100,000. We present a microfluidic 'megapixel' digital PCR device that uses surface tension–based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm−2. This device achieves a dynamic range of 107, single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.Keywords
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