BEAMing up for detection and quantification of rare sequence variants
- 23 January 2006
- journal article
- Published by Springer Nature in Nature Methods
- Vol. 3 (2) , 95-97
- https://doi.org/10.1038/nmeth850
Abstract
BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.Keywords
This publication has 12 references indexed in Scilit:
- Detection and quantification of mutations in the plasma of patients with colorectal tumorsProceedings of the National Academy of Sciences, 2005
- Accurate Multiplex Polony Sequencing of an Evolved Bacterial GenomeScience, 2005
- Genome sequencing in microfabricated high-density picolitre reactorsNature, 2005
- Quantification of random genomic mutationsNature Methods, 2005
- In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probesNature Methods, 2004
- LigAmp for sensitive detection of single-nucleotide differencesNature Methods, 2004
- Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variationsProceedings of the National Academy of Sciences, 2003
- A sensitive scanning technology for low frequency nuclear point mutations in human genomic DNANucleic Acids Research, 2000
- Mutation detection and single-molecule counting using isothermal rolling-circle amplificationNature Genetics, 1998
- Fidelity of DNA synthesis by the Thermus aquaticus DNA polymeraseBiochemistry, 1988