Quantitative Determination of Creatine Kinase Isoenzyme Catalytic Concentrations in Serum Using Immunological Methods
- 1 January 1977
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 15 (3) , 131-138
- https://doi.org/10.1515/cclm.1977.15.1-12.131
Abstract
For the determination of creatine kinase isoenzyme catalytic concentrations in serum two methods based on immunological reactions are presented: One method uses inhibiting antibodies, which selectively block the activity of creatine kinase M subunits ("Inhibition Test"). This test is used for routine measurements of creatine kinase MB catalytic concentration; Another method uses precipitating antibodies, which allows a quantitative differentiation of creatine kinase isoenzymes MM, MB and BB ("Precipitation Test"). This test is used as a control for the Inhibition Test for the possible presence of creatine kinase BB activities in doubtful cases. Procedures, specificity, correlation and application of these methods are discussed.Keywords
This publication has 18 references indexed in Scilit:
- Determination of creatine kinase isoenzyme MB activity in serum using immunological inhibition of creatine kinase M subunit activity activity kinetics and diagnostic significance in myocardial infarctionClinica Chimica Acta; International Journal of Clinical Chemistry, 1976
- Isoenzyme der Kreatinkinase bei extrakardialen Erkrankungen und nach diagnostischen und therapeutischen EingriffenDeutsche Medizinische Wochenschrift (1946), 1976
- Bestimmung der Aktivit t von Creatinkinase MB im Serum unter Verwendung inhibierender Antik rperKlinische Wochenschrift, 1976
- Quantitation of creatine kinase isoenzymes in human tissues and sera by an immunological methodClinica Chimica Acta; International Journal of Clinical Chemistry, 1975
- Immunologischer Nachweis von Creatinkinase-MB im Serum beim MyokardinfarktKlinische Wochenschrift, 1975
- Quantification of serum creatine phosphokinase isoenzyme activityThe American Journal of Cardiology, 1974
- Kinetic Properties of the Isoenzymes of Human Creatine PhosphokinaseProceedings of the National Academy of Sciences, 1974
- Separation of tissue and serum creatine kinase isoenzymes on polyacrylamide gel slabsClinica Chimica Acta; International Journal of Clinical Chemistry, 1972
- Studies on adenosine triphosphate transphosphorylasesArchives of Biochemistry and Biophysics, 1972
- A method allowing the quantitation of serum creatine kinase isoenzymesClinica Chimica Acta; International Journal of Clinical Chemistry, 1972