Effect of Su-4885 on Steroid 18-Hydroxylation in the Mouse Adrenalin Vitro1

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Abstract
The conversion of progesterone-4-C14 by quartered mouse adrenal glands to 18-oxygenated steroids was studied in vitro following incubation with the inhibitor, 2-methyl-1, 2-bis-(3-pyridyl)-l-propa-none (Su-4885). The conversion of progesterone to aldosterone and 18-hydroxycorticosterone was decreased to less than one half of the controls at concentrations of Su-4885 (5 X 10-6M) lower than that required for the equivalent inhibition of corticosterone formation. Aldosterone and 18-hydroxycorticosterone were identified by the mobilities of the labeled steroids and their derivatives and by the constancy of radioactivity in different chromatographic systems.