Microarray‐based genotyping for blood groups: comparison of gene array and 5′‐nuclease assay techniques with human platelet antigen as a model
- 6 April 2005
- journal article
- Published by Wiley in Transfusion
- Vol. 45 (5) , 654-659
- https://doi.org/10.1111/j.1537-2995.2005.04318.x
Abstract
Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of a parallel developed third-generation technique (TaqMan assay, Applied Biosystems). Both techniques were validated by use of a unique panel of 71 blinded DNA samples containing at least 15 aa, bb, and ab genotypes for the HPA-1, -2, -3, -5, and-15 systems. Unambiguous and concordant results were obtained with both techniques for all samples. The data presented here validate the use of microarray for large-scale SNP typing for clinically relevant blood group alloantigens.Keywords
This publication has 16 references indexed in Scilit:
- Parallel Genotyping of Over 10,000 SNPs Using a One-Primer Assay on a High-Density Oligonucleotide ArrayGenome Research, 2004
- Fluorescence Polarization in Homogeneous Nucleic Acid Analysis II: 5′-Nuclease AssayGenome Research, 2001
- Genome-wide Detection of Allelic Imbalance Using Human SNPs and High-density DNA ArraysGenome Research, 2000
- 3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperaturesNucleic Acids Research, 2000
- Allelic discrimination using fluorogenic probes and the 5′ nuclease assayGenetic Analysis: Biomolecular Engineering, 1999
- HPA genotyping by PCR sequence‐specific priming (PCR–SSP): a streamlined method for rapid routine investigationsTransfusion Medicine, 1997
- Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysisNature Genetics, 1996
- Rapid determination of platelet alloantigen genotypes by polymerase chain reaction using allele‐specific primersTransfusion, 1994
- HPA‐1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP): a rapid and simple techniqueBritish Journal of Haematology, 1993
- Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.Proceedings of the National Academy of Sciences, 1985