A method for the simultaneous isolation of Factor X and prothrombin from bovine plasma
- 1 April 1973
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 131 (4) , 781-789
- https://doi.org/10.1042/bj1310781
Abstract
1. A method is described for the simultaneous isolation of both Factor X and prothrombin from bovine plasma. The proteins are adsorbed on and eluted from barium sulphate and chromatographed on DEAE-Sephadex and are finally purified by rechromatography on DEAE-Sephadex. 2. The proteins can be purified in 48h from the collection of the blood and the method can be used to process large volumes of plasma. 3. The prothrombin has a molecular weight of 70300; the Factor X, on the other hand, is polydisperse, with most of the protein (86%) having a molecular weight of 56000.Keywords
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