Separation of Deoxyribonucleotides Using Ion Suppression High Performance Liquid Chromatography with a Reversed-Phase Column
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 5 (11) , 2085-2093
- https://doi.org/10.1080/01483918208067619
Abstract
Ion suppression-reversed phase high performance liquid chromatography, using 0.6 M ammonium dihydrogen phosphate as eluent, produces base-line separations of deoxyribonucleotides. The effects of pH and ionic strength are described. This isochratic system is simple, reproducible and fast, requiring less than 30 min for a complete separation, and is suitable for in vitro studies.Keywords
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