Continuous production of bacteriocins, brevicin, nisin and pediocin, using calcium alginate‐immobilized bacteria
- 1 December 1995
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 79 (6) , 671-676
- https://doi.org/10.1111/j.1365-2672.1995.tb00953.x
Abstract
Bacteriocins including brevicin 286, nisin and pediocin PO2 have been produced successfully with immobilized cells of Lactobacillus brevis VB286, Lactococcus lactis subsp. lactis and Pediococcus acidilactici PO2 respectively encapsulated in calcium alginate beads. The beads encapsulating the bacteriocin‐producing cells were loaded into a column, and continuously supplied with fresh medium at a flow rate of 1 bed volume per 20 min. The concentrations of the three bacteriocins produced in the eluents were at least as high as those obtained from conventional free‐cell batch fermentations.Keywords
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