Immunoenzymetric Assay of Mouse and Human Cytokines Using NIP‐Labeled Anti‐Cytokine Antibodies
Open Access
- 1 March 1995
- journal article
- unit
- Published by Wiley in Current Protocols in Immunology
- Vol. 13 (1) , 6.20.1-6.20.15
- https://doi.org/10.1002/0471142735.im0620s13
Abstract
This unit describes a general method for immunoenzymetric assay of mouse and human cytokines. The technique is based on use of two anti-cytokine monoclonal antibodies (MAbs), each specific for a spatially distinct determinant on the cytokine. One of these is a coating antibody, and the other is a derivatized detecting antibody. A support protocol describes chemical labeling of anti-cytokine monoclonal IgG antibodies with the NIP hapten group to produce the detecting antibody. Another support protocol describes a method for producing horseradish peroxidase (HRPO)-conjugated J4, a rat IgG1 anti-NIP monoclonal antibody that confers the anti-NIP specificity to the HRPO detection system used in the immunoenzymetric assay. The method described in this unit has allowed successful measurement of different cytokines in a wide variety of clinical samples including conditioned medium, serum and plasma, ascites, amniotic fluid, and bronchoalveolar lavage fluid.Keywords
This publication has 13 references indexed in Scilit:
- Heterogeneity of antigen-specific CD4+T cell clones from a patient with Schistosomiasis mansoniParasite Immunology, 1994
- Interleukin-10 in amniotic fluid at midtrimester: Immune activation and suppression in relation to fetal growthAmerican Journal of Obstetrics and Gynecology, 1994
- Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen: Cytokine detection by immunoassay and intracellular immunostrainingJournal of Immunological Methods, 1993
- Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancersCytokine, 1992
- Strategies of Anti‐Cytokine Monoclonal Antibody Development: Immunoassay of IL‐10 and IL‐5 in Clinical SamplesImmunological Reviews, 1992
- Immediate and Late Airway Response of Allergic Rhinitis Patients to Segmental Antigen Challenge: Characterization of Eosinophil and Mast Cell MediatorsAmerican Review of Respiratory Disease, 1991
- Interleukin-5 and the posttreatment eosinophilia in patients with onchocerciasis.Journal of Clinical Investigation, 1991
- Production of Interferon-Gamma during Infection of Mice with Plasmodium chabaudi chabaudiImmunobiology, 1989
- Data Reduction Techniques for ImmunoassayPublished by Elsevier ,1987
- Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities.The Journal of Experimental Medicine, 1984