A selective inhibitor of cyclic AMP‐dependent protein kinase, N‐[2‐bromocinnamyl(amino)ethyl]‐5‐isoquinolinesulfonamide (H‐89), inhibits phosphatidylcholine biosynthesis in HeLa cells

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Abstract
In this study, we report that the potent and selective inhibitor of cyclic AMP‐dependent protein kinase, N‐[2‐bromocinnamyl(amino)ethyl]‐5‐isoquinolinesulfonamide (H‐89) interferes with the incorporation of choline into phosphatidylcholine in HeLa cells. Treatment of cells with 10 μM H‐89 for 1 h decreases the phosphatidylcholine biosynthesis by 50%. This inhibition is prevented by simultaneous addition of 10 μM forskolin, while the choline uptake itself is not affected by H‐89.

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