GENETIC ANALYSIS OF SEGREGANTS FROM HETEROKARYONS OF STREPTOMYCES COELICOLOR

Publisher Website
Google Scholar
Abstract
Heterokaryons of Streptomyces coelicolor, formed by the interaction of complementary auxotrophs, were generally stable. Rare segregants were isolated, which usually resembled one of the parental types. Nonparental auxotrophic segregants were also found and fell into two classes: (a) anomalous heterokaryons with at least one nutritional requirement from each parent, and (b) auxotrophs with nutritional requirements not recognized in either parent. In the former class both parental genomes were present but in imbalanced ratios (ratios of parental nuclei between 1:10 and 1:1000 instead of 1:10 - 1:1 as in normal heterocaryons.) The class of segregants that displayed a new auxotrophic character probably arose by mutation.

This publication has 16 references indexed in Scilit: