Protein phosphorylation in isolated mitochondria and the effects of protein kinase C
- 5 May 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 200 (1) , 161-164
- https://doi.org/10.1016/0014-5793(86)80530-0
Abstract
When isolated intact rat liver mitochondria are incubated with [γ‐32P]ATP the major phosphorylated proteins are those of 47 and 36 kDa. Phosphorylation of the 47 kDa protein, but not of the 36 kDa protein, is inhibited by carboxyatractyloside, an inhibitor of mitochondrial ATP uptake, while phosphorylation of the 36 kDa protein is inhibited by various uncouplers and an inhibitor of mitochondrial respiration. Addition of purified protein kinase C to the isolated mitochondria leads to the phosphorylation of 69, 37 and 17 kDa proteins. As with other substrates for protein kinase C, phosphorylation of these proteins is dependent on Ca2+ and markedly stimulated by various tumor promoters.Keywords
This publication has 16 references indexed in Scilit:
- Cellular uptake and localization of fluorescent derivatives of phorbol ester tumor promotersBiochemical and Biophysical Research Communications, 1985
- Intracellular localization of 12-O-3-N-dansylamino TPA in C3H/10T1/2 mouse cell lineCarcinogenesis: Integrative Cancer Research, 1985
- Adrenocortical cytochrome P-450 responsible for cholesterol side chain cleavage (P-450scc) is phosphorylated by the calcium-activated, phospholipid-sensitive protein kinase (protein kinase C)Biochemical and Biophysical Research Communications, 1984
- Further characterization of tumor-promoter-mediated activation of protein kinase CBiochemical and Biophysical Research Communications, 1984
- Phosphorylation of a 16‐kDa protein by diacylglycerol‐activated protein kinase C in vitro and by vasopressin in intact hepatocytesFEBS Letters, 1984
- Phosphorylation of mitochondrial membrane proteins: effect of the surface potential on monoamine oxidaseFEBS Letters, 1983
- Endogenous Protein Phosphorylation in Rat Brain Mitochondria: Occurrence of a Novel ATP‐Dependent Form of the Autophosphorylated Enzyme Succinyl‐CoA SynthetaseJournal of Neurochemistry, 1982
- Inhibition of respiration by a phorbol ester tumor promoter in murine cultured cellsBiochemical and Biophysical Research Communications, 1982
- Protein kinase activity and endogenous phosphorylation in subfractions of rat liver mitochondriaBiochimica et Biophysica Acta (BBA) - General Subjects, 1979