Intracellular localization of 12-O-3-N-dansylamino TPA in C3H/10T1/2 mouse cell line
- 1 March 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 6 (3) , 433-439
- https://doi.org/10.1093/carcin/6.3.433
Abstract
The fluorescent 12-O-tetradecanoylphorbol-13-acetate (TPA) analogue dansyl-TPA allowed the distribution of fluorescent molecules to be visualized in living mouse fibroblasts. The entry of dansyl-TPA into cells occurred within millisecond time scale and was found largely energy independent. Dansyl-TPA was shown to stain the endoplasmic reticulum, Golgi apparatus, mitochondria and nuclear membrane. In addition, dansyl-TPA fluorescence was found to parallel that obtained with rhodamine-conjugated anti-α tubulin. The intracellular location of the dansyl-TPA fluorescence can be taken into account to explain the pleiotropic action of TPA in the cells.This publication has 22 references indexed in Scilit:
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