ASSIGNMENT OF ALLOTYPES G1M(A+) AND G1M(A-) AT THE GENOMIC LEVEL BY POLYMERASE CHAIN-REACTION ANALYSIS
- 1 January 1990
- journal article
- research article
- Vol. 7 (4) , 205-212
Abstract
A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding C(H)3 of gamma-chains, followed by dot-blot hybridization of radioactivity labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.Keywords
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