Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promotersybtAandirp6ofYersinia enterocolitica
Open Access
- 1 September 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 250 (1) , 27-32
- https://doi.org/10.1016/j.femsle.2005.06.040
Abstract
Genes of Yersiniae spp. involved in production of the siderophore yersiniabactin are located on the high-pathogenicity island (HPI). Their transcription is controlled by the AraC/XilS-like transcriptional regulator YbtA encoded within the HPI. YbtA-regulated divergent and overlapping ybtA and irp6 promoters contain three YbtA binding sites, RS1, RS2 and RS3. Deleting RSs systematically and using ybtA and irp6 transcriptional fusions, we determined that different modes of YbtA binding are responsible for activation of irp6 and repression of ybtA. Based on these data, we propose a model of irp6 and ybtA promoter regulation.Keywords
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