Abstract
Trypsin was prepared free from enterokinase and the pre-stage of the activator. The course of the heat-inactiyation purified trypsin followed the unimolecular equation. The purified trypsin had an optimum stability at about pH 6.5. The critical increment of the heat-inactivation of trypsin was about 40,000 calories per molar unit of enzyme at pH 5-9.

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