Development of multi‐residue methodology for the HPLC determination of veterinary drugs in animal tissues

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Abstract
The application of solid‐phase extraction and HPLC with UV‐diode array detection to the multi‐residue determination of veterinary drugs is described. A two‐stage SPE clean‐up was employed, using C18 and silica cartridges. HPLC analysis was carried out on a base‐deactivated C8 column using gradient elution systems at two pH values. The procedure establishes the basis of a method for routine screening of pig kidney samples for some sulphonamides, benzimidazoles, nitroimidazoles and nitrofurans at concentrations at or below the UK Maximum Residue Limits (MRLs). Limits of detection of 2–18 μg/kg could be achieved for these analytes at recoveries of 40–70%. UV spectra measured on‐line were used for confirmation of peak identities at these concentrations. The possibility of extension of this procedure to a wider range of analytes is discussed.