Ribosome specificity of archaebacterial elongation factor 2
- 2 May 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 155 (1) , 173-177
- https://doi.org/10.1016/0014-5793(83)80233-6
Abstract
Polyphenylalanine synthesis with ribosomes and two separated, partially purified elongation factors (EF) was measured in cell-free systems from the archaebacteria Thermoplasma acidophilum and Methanococcus vannielii, in an eukaryotic system from rat liver and an eubacterial one with Escherichia coli ribosomes and factors from Thermus thermophilus. By substitution of heterologous EF-2 or EF-G, respectively, for the homologous factors, ribosome specificity was shown to be restricted to factors from the same kingdom. In contrast EF-1 from T. thermophilus significantly cooperated with ribosomes from T. acidophilum.Keywords
This publication has 17 references indexed in Scilit:
- Purification of Euglena EF-1L: A cytoplasmic factor that also functions on procaryotic and organellar ribosomesArchives of Biochemistry and Biophysics, 1982
- The inhibition of eucaryotic protein synthesis by procaryotic elongation factor TuBiochemical and Biophysical Research Communications, 1981
- Archaebacterial elongation factor is ADP-ribosylated by diphtheria toxinNature, 1980
- The Phylogeny of ProkaryotesScience, 1980
- Elongation factors EF-G from E. coli and mammalian mitochondria are not functionally interchangeableBiochemical and Biophysical Research Communications, 1979
- [56] Enzymic binding of aminoacyl-tRNA to Escherichia coli ribosomes using modified tRNA species and tRNA fragmentsPublished by Elsevier ,1979
- ArchaebacteriaJournal of Molecular Evolution, 1978
- Ribosome Specificity of Protein Synthesis in VitroProgress in Nucleic Acid Research and Molecular Biology, 1970
- Aminosäure-Transferenzyme aus LeberBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967