Secretion of a nerve growth factor by mouse neuroblastoma cells in culture.
- 1 May 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (5) , 1895-1898
- https://doi.org/10.1073/pnas.72.5.1895
Abstract
Analyses of supernatant solutions from mouse C1300 neuroblastoma cultures by two independent immunoassays reveal that these cells secrete a factor which is immunochemically similar to mouse submaxillary gland nerve growth factor. The neuroblastoma factor is also biologically active in inducing neurite outgrowth from embryonic sensory ganglia-an effect that is completely blocked by specific antibody to nerve growth factor. Neuroblastoma cells are known to be functionally responsive to nerve growth factor, and the observation that they secrete a molecule like it may mean that these cells require or utilize the factor during growth in culture.Keywords
This publication has 15 references indexed in Scilit:
- Cyclic expression of a growth conditioning factor (MGF) on the cell surfaceNature, 1974
- Effect of Nerve Growth Factor on the Expression of Colchicine-Binding Activity and 14-3-2 Protein in an Established Line of Human NeuroblastomaProceedings of the National Academy of Sciences, 1974
- SPECIFIC BINDING OF NERVE GROWTH FACTOR (NGF) BY MURINE C 1300 NEUROBLASTOMA CELLSThe Journal of Experimental Medicine, 1974
- Nerve Growth Factor in Rat Glioma CellsProceedings of the National Academy of Sciences, 1974
- Synthesis of Nerve Growth Factor by L and 3T3 Cells in CultureProceedings of the National Academy of Sciences, 1974
- Interaction of Nerve Growth Factor with Surface Membranes: Biological Competence of Insolubilized Nerve Growth FactorProceedings of the National Academy of Sciences, 1973
- Binding of Nerve Growth Factor Receptor in Sympathetic GangliaProceedings of the National Academy of Sciences, 1973
- Glia-Induced Morphological Differentiation in Neuroblastoma CellsProceedings of the National Academy of Sciences, 1973
- Replacement of Nerve-Growth Factor by Ganglionic Non-Neuronal Cells for the Survival In Vitro of Dissociated Ganglionic NeuronsProceedings of the National Academy of Sciences, 1972
- THE NERVE GROWTH FACTOR: PURIFICATION AS A 30,000-MOLECULAR-WEIGHT PROTEINProceedings of the National Academy of Sciences, 1969