Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.
- 1 May 1976
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (5) , 1476-1479
- https://doi.org/10.1073/pnas.73.5.1476
Abstract
The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.Keywords
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