Evidence for T–DNA Mediated Gene Targeting to Tobacco Chloroplasts
- 1 November 1991
- journal article
- research article
- Published by Springer Nature in Bio/Technology
- Vol. 9 (11) , 1103-1105
- https://doi.org/10.1038/nbt1191-1103
Abstract
The integration of foreign DNA into plant cells by Agrobacterium mediated transformation is random and normally directed at the nucleus. Here we present evidence that such transformation can be used to introduce foreign genes into higher plant chloroplasts by site–specific homologous recombination. A binary vector was made in vitro that included chloroplast ribosomal DNA (rDNA) within T–DNA borders, and transgenic tobacco plants obtained using this construct were analyzed for the targeted recombination by hybridization, polymerase chain reaction (PCR) and DNA sequencing.Keywords
This publication has 16 references indexed in Scilit:
- Homologous recombination in plant cells after Agrobacterium-mediated transformation.Plant Cell, 1990
- Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome.Plant Cell, 1989
- Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genesNature, 1988
- Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the texas male-sterile cytoplasmCell, 1986
- An efficient procedure for isolation of nuclei from plant protoplastsProtoplasma, 1985
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cellsNature, 1985
- Inheritance of Functional Foreign Genes in PlantsScience, 1984
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983