Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae.
- 1 October 1986
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 6 (10) , 3559-3562
- https://doi.org/10.1128/mcb.6.10.3559
Abstract
A plasmid containing the denV gene from bacteriophage T4, under the control of the yeast alcohol dehydrogenase I (ADC1) promoter, conferred a substantial increase in UV resistance of the UV-sensitive Saccharomyces cerevisiae mutants rad1-2 and rad3-2. The UV resistance of the denV+ yeast cells was cell cycle dependent and correlated well with the level of the denV gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-DNA glycosylate activity.This publication has 25 references indexed in Scilit:
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