Versatile and Stable Recording Spectrofluorometer for the Measurement of Corrected Spectra

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Abstract
The design and performance of a versatile automatic recording spectrofluorometer are described. The instrument has a modular design and is capable of measuring fluorescence excitation and emission spectra, polarization of fluorescence, and phosphorescence spectra, each as a function of temperature and pH. Both right angle and frontal illumination may be used. A split beam optical system combined with an automatic gain control (AGC) amplifier assures long term stability and freedom from the effects of light source fluctuations. Corrected emission spectra in energy units are obtained automatically by the use of an adjustable function generator (Vernistat). A quantum counter used in conjunction with a second function generator and the AGC amplifier permits recording of true excitation spectra.