A New Extractive-spectrophotometric Method for the Determination of Ketoconazole from Pharmaceutical Preparations
- 1 December 1998
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 31 (15) , 2691-2705
- https://doi.org/10.1080/00032719808005336
Abstract
An extraction-spectrophotometric method for the determination of trace amounts of ketoconazole is described. Ketoconazole is effectively extracted as a 1:1 ion-pair complex with picric acid at pH 2.5 into chloroform, followed by spectrophotometric determination at 410 nm. Beer's law is obeyed over the range 1-58 μg ml−1 and the Sandell sensitivity for 0.001 absorbance unit is 57 ng cm−2. The method has a wide range and is simple, rapid and sensitive. The procedure was applied to the determination of ketoconazole in tablets and creams as well as its recovery from a blood serum sample.Keywords
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