Two Distinct Immunogenic Epitopes on the α Chain of Human T Cell Antigen Receptor

Publisher Website
Google Scholar
Abstract
Monoclonal antibodies (MAb) to human T Cell Antigen Receptor (TCR) have been used to study the structure and function of TCR. Using purified α/β heterodimeric protein, we have generated two MAb against human TCR α protein. The two MAb, αF1 and αF2, recognized amino acid residues 141-159 and 212-231 of the constant region of the α chain TCR. Although neither MAb reacted with viable T cells, both antibodies immunoprecipitated TCR α/β heterodimer from HPB-ALL, Jurkat, PBL and a 32 kDa in vitro translation product of α chain cDNA. These antibodies have been shown to be useful in the immunohistochemical staining of human tissues. These two MAb, together with other anti-framwork MAb to TCR, should provide valuable reagents in the study of TCR and clinical classification of T cell lineage neoplasms.