A direct radioimmunoassay for aldosterone in unextracted serum and plasma.
Open Access
- 1 December 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 27 (12) , 2019-2021
- https://doi.org/10.1093/clinchem/27.12.2019
Abstract
We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactivates the enzyme before radioimmunoassay. Alternatively, EDTA may be used to inactivate the enzyme. The antibody-bound fraction is then precipitated with polyethylene glycol and isolated by centrifugation. This easy method eliminates extraction and purification and gives accurate and reliable results. The total time required for 100 estimations, including counting time, is about 6 h.This publication has 4 references indexed in Scilit:
- Characteristics of Aldosterone Binding in Human Plasma*Journal of Clinical Endocrinology & Metabolism, 1980
- The Development and Application of a Direct Radioimmunoassay for Plasma Aldosterone Using125I-Labeled Ligand—Comparison of Three Methods*†Journal of Clinical Endocrinology & Metabolism, 1978
- A Non-Chromatographic Non-Extraction Radioimmunoassay for Serum AldosteroneJournal of Clinical Endocrinology & Metabolism, 1977
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951