Cloning and functional characterization of LCR-F1: a bZIP transcription factor that activates erythroid-specific, human globin gene expression
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (12) , 2383-2391
- https://doi.org/10.1093/nar/22.12.2383
Abstract
DNase I hypersensitive site 2 (HS 2) of the human beta-globin Locus Control Region (LCR) directs high level expression of the beta-globin gene located 50 kilobases downstream. Experiments in cultured cells and in transgenic mice demonstrate that duplicated AP1-like sites in HS 2 are required for this powerful enhancer activity. A cDNA clone encoding a basic, leucine-zipper protein that binds to these sites was isolated and designated Locus Control Region-Factor 1 (LCR-F1). This protein is a member of a new family of regulatory factors that contain a 63 amino acid 'CNC domain' overlapping the basic region. This domain is approximately 70% identical in the Drosophila Cap N Collar (CNC) protein, NF-E2 and LCR-F1. LCR-F1 transactivates an HS 2/gamma-globin reporter gene over 170-fold in transient transfection experiments specifically in erythroid cells. These results suggest that LCR-F1 may be a critical factor involved in LCR-mediated, human globin gene expression.Keywords
This publication has 43 references indexed in Scilit:
- Developmental regulation of human β-globin gene transcription: a switch of loyalties?Trends in Genetics, 1993
- A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effect in DrosophilaCell, 1993
- Transcriptional regulation of multigene loci: multilevel controlTrends in Genetics, 1993
- Erythroid transcription factor NF-E2 is a haematopoietic-specific basic–leucine zipper proteinNature, 1993
- Human globin locus activation region (LAR): role in temporal controlTrends in Genetics, 1990
- Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells.Genes & Development, 1990
- In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.Genes & Development, 1988
- The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding ProteinsScience, 1988
- Molecular cloning of an enhancer binding protein:Isolation by screening of an expression library with a recognition site DNACell, 1988
- Position-independent, high-level expression of the human β-globin gene in transgenic miceCell, 1987