Identification of cell surface receptors for the Act-2 cytokine.
Open Access
- 1 July 1990
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 172 (1) , 285-289
- https://doi.org/10.1084/jem.172.1.285
Abstract
We have identified cell surface receptors for Act-2, a secreted protein expressed upon activation of T cells, B cells, and monocytes. Although 125I-Act-2 showed little, if any, specific binding to resting peripheral blood lymphocytes (PBL) receptors were readily detected on PHA/PMA-activated PBL and a variety of cell lines including MT-2, HL60, DMSO differentiated HL60, HeLa, and K562 cells. The equilibrium dissociation constant (Kd) is 3-12 nM for MT-2, K562, and PBL activated with PHA/PMA for 40-80 h. We have also identified a rabbit polyclonal antiserum that can block Act-2 binding to its receptors. The ability to detect specific Act-2 receptors and the development of a blocking antiserum should prove valuable in efforts to molecularly clone the Act-2 receptor and to dissect the biological actions of Act-2.Keywords
This publication has 13 references indexed in Scilit:
- Identification and characterization of an inhibitor of haemopoietic stem cell proliferationNature, 1990
- A novel polypeptide secreted by activated human T lymphocytes.The Journal of Immunology, 1989
- Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors.The Journal of Immunology, 1989
- A family of small inducible proteins secreted by leukocytes are members of a new superfamily that includes leukocyte and fibroblast-derived inflammatory agents, growth factors, and indicators of various activation processes.The Journal of Immunology, 1989
- Identification, cloning, and characterization of an immune activation gene.Proceedings of the National Academy of Sciences, 1988
- Automated synthesis and use of N‐chloroacetyl‐modified peptides for the preparation of synthetic peptide polymers and peptide‐protein immunogensInternational Journal of Peptide and Protein Research, 1987
- The interleukin 2 receptor. Functional consequences of its bimolecular structure.The Journal of Experimental Medicine, 1987
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.Proceedings of the National Academy of Sciences, 1986
- LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry, 1980