Identification, cloning, and characterization of an immune activation gene.
- 1 December 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (24) , 9704-9708
- https://doi.org/10.1073/pnas.85.24.9704
Abstract
We have identified an immune activation gene, denoted Act-2, by differential hybridzation screening of an activated T-cell library. The gene is induced rapidly after T-cell activation with phytohemagglutinin, B-cell activation with Staphylococcus aureus Cowan I, and monocyte activation with lipopolysaccharide. We have isolated a cDNA containing the full-length coding region. The deduced amino acid sequence predicts an open reading frame of 92 amino acids, including a very hydrophobic N terminus, which by weight matrix score is predicted to be a signal pepetide. Using a baculovirus expression system, we have shown that this gene encodes a secreted product. It is therefore possible that Act-2 represents a newly discovered cytokine.This publication has 19 references indexed in Scilit:
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