Toxicological assessment of biodegraded pentachlorophenol: Microtox and fish embryos

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Abstract
A Gram-negative bacterium,Pseudomonas sp. strain SR3, was isolated from soil at a former wood treatment plant in north central Florida. The ability of this bacterium to degrade pentachlorophenol (PCP) was confirmed by growing cells in a basal salts medium in which PCP was the only source of carbon and energy. Degradation from a measured concentration of 39–40 μg PCP/ml to 0.0006 μg PCP/ml was observed within 120 h of incubation in the presence of PCP-induced cells ofPseudomonas sp. strain SR3. The initial cell density in these cultures was 6 x 106 cfu/ml. Microtox® 5 min EC50 toxicity tests revealed that aqueous solutions of PCP, measured concentrations 39–40 p μ/ml were toxic but that final biodegraded samples, 0.0006 μ/g PCP/ml were nontoxic. However, bioassays with embryonic inland silversides,Menidia beryllina, showed that the biodegraded samples were embryotoxic or teratogenic. Water containing added PCP at concentrations up to 30 times higher than measured in the final biodegraded samples was less toxic/teratogenic. These results indicate that while biodegradation of PCP was nearly complete, intermediate metabolites of the degradation process or undegraded impurities in PCP were toxic or teratogenic. Thus, theM. beryllina bioassay allows extremely sensitive assessment of toxicity associated with biodegraded environmental pollutants and may be a useful criterion for determining whether bioremediated water or soil is safe for discharge back into the environment.