Antiviral activity of primate γδ T lymphocytes isolated by magnetic cell sorting

Abstract
The exposure of human or rhesus monkey peripheral blood mononuclear cells (PBMCs) to interleukin 2 (IL‐2) in vitro resulted in a selective outgrowth of γδ lymphocytes. Using positive selection by monoclonal antibodies and magnetic beads, γδ T lymphocytes were isolated from these cultures. Without priming by viral antigens, the purified γδ T lymphocytes lyse immunodeficiency virus‐infected cells substantially better than the uninfected counterparts.