An Improved Method for the Extraction of Cholesterol and Phospholipids From the Bovine Spinal Cord
- 31 December 1977
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 8 (2-3) , 113-118
- https://doi.org/10.1080/00327487808069053
Abstract
A pharmacopoeial grade of cholesterol and a separate phospholipid fraction can be obtained by this simple extraction procedure of bovine spinal cord. The tissue is collected in 5% (Wt/V) formalin. It is then separated from the formalin solution, minced and dried in a hot-air oven at 105°C. Total lipids are extracted with petroleum ether. The cholesterol is separated from the phospholipids by extraction with a mixture of petroleum ether and acetone. The method allows for not only the recovery of the phospholipids but also a high yield of cholesterol without using saponification or tedious purification procedures.This publication has 2 references indexed in Scilit:
- A Simple and Rapid Method for the Isolation of Cholesterol from Spinal Cord of CattlePreparative Biochemistry, 1976
- Eine schnelle Methode zur Cholesterindarstellung aus Hirn.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1936