The gene for a tRNA modifying enzyme, m5U54-methyltransferase, is essential for viability in Escherichia coli.
- 1 May 1992
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (9) , 3995-3998
- https://doi.org/10.1073/pnas.89.9.3995
Abstract
One of the most abundant modified nucleosides in tRNA is 5-methyluridine (m5U or rT, ribothymidine). The enzyme tRNA(m5U54)methyltransferase [S-adenosyl-L-methionine:tRNA (uracil-5-)-methyltransferase, EC 2.1.1.35] (the trmA gene product) catalyzes S-adenosylmethionine-dependent methylation of the uracil in position 54 (T psi C loop) in all Escherichia coli tRNAs to form m5U. Hitherto no modified nucleoside in tRNA has been shown to be essential for growth, although their importance in fine tuning the function of tRNA is well established. In this paper, we show that the structural gene trmA is essential for viability, although the known catalytic activity of the tRNA(m5U54)methyltransferase is not.Keywords
This publication has 28 references indexed in Scilit:
- Codon recognition patterns as deduced from sequences of the complete set of transfer RNA species in Mycoplasma capricolum: Resemblance to mitochondriaPublished by Elsevier ,2004
- Efficient introduction of cloned mutant alleles into the Escherichia coli chromosomeJournal of Bacteriology, 1991
- The trmA promoter has regulatory features and sequence elements in common with the rRNA P1 promoter family of Escherichia coliJournal of Bacteriology, 1991
- The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutantsNature, 1989
- Non-autogenous control of ribosomal protein synthesis from the trmD operon in Escherichia coliJournal of Molecular Biology, 1988
- The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic libraryCell, 1987
- TRANSFER RNA MODIFICATIONAnnual Review of Biochemistry, 1987
- Defects in modification of cytoplasmic and mitochondrial transfer RNAs are caused by single nuclear mutationsCell, 1982
- On the Role of Ribosylthymine in Prokaryotic tRNA FunctionEuropean Journal of Biochemistry, 1981
- Isolation and properties of a transfer ribonucleic acid deficient in ribothymidineBiochemistry, 1970