Microsatellite hybrid capture technique for simultaneous isolation of various STR markers.
Open Access
- 1 July 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 6 (7) , 646-649
- https://doi.org/10.1101/gr.6.7.646
Abstract
The microsatellite hybrid capture technique was designed to enrich simultaneously for various microsatellite repeats from a genomic clone. It is illustrated in this report that different repeat motifs, including polymorphic ones, can be efficiently isolated in a single experiment. In principle, this technique can be applied to any type of genomic clones to facilitate the isolation of informative markers for fine mapping of subchromosomal regions of interest, and for linkage and association studies of candidate genes for which no polymorphic markers are available yet.Keywords
This publication has 20 references indexed in Scilit:
- Molecular and linkage analysis of type-1 protein phosphatase catalytic beta-subunit gene: lack of evidence for its major role in insulin resistance in Pima IndiansDiabetologia, 1995
- A simple method for rapid isolation of microsatellites from yeast artificial chromosomesMolecular and Cellular Probes, 1995
- MATS: a rapid and efficient method for the development of microsatellite markers from YACsGenomics, 1995
- Dinucleotide repeat polymorphism adjacent to CDKN2Human Molecular Genetics, 1994
- The 1993–94 Généthon human genetic linkage mapNature Genetics, 1994
- Integrated human genome–wide maps constructed using the CEPH reference panelNature Genetics, 1994
- Isolation of human simple repeat loci by hybridization selectionHuman Molecular Genetics, 1994
- Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers.Proceedings of the National Academy of Sciences, 1994
- Pima Indians as a model to study the genetics of NIDDMJournal of Cellular Biochemistry, 1992
- Survey of human and rat microsatellitesGenomics, 1992