SEPARATION OF HUMAN THYROTROPIN FROM PLASMA BY ION EXCHANGE CHROMATOGRAPHY*

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Abstract
Thyrotropic activity was recovered completely from dialyzed mixtures of 7–35 ml. of human plasma and crude human pituitary preparations by chromatography on pH 7.2–7.8 Amberlite IRC-50 columns, eluted with a gradient to 2 M sodium chloride. This procedure was successful over a range of 0.08–17u.s.p. milliunits per milliliter of plasma and separated thyrotropin from the nondiatyzable substances in plasma interfering with the bioassay method used. Endogenous thyrotropic activity (0.2 milliunit per milliliter) was measured in the plasma of a myxedematous patient by this method. The active fraction was in the same location in the elution chromatogram as the activity recovered from mixtures of human plasma and crude human pituitary thyrotropin, or from the latter alone.