Qualitative and quantitative studies on IgG2 globulins in individual human sera with an antiserum capable of differentiating between Gm(n+) and Gm(n-) proteins.

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  • 1 May 1973
    • journal article
    • Vol. 14  (1) , 127-39
Abstract
A monkey antiserum was obtained by immunization with Gm(n+) IgG2 proteins. After it had been rendered specific for the IgG2 subclass, it was shown to contain antibodies of two specificities, i.e. anti-γ2 and anti-Gm(n). Precipitation and haemagglutination experiments, using unabsorbed antiserum and antiserum absorbed with Gm(n+) or Gm(n−) proteins, made it clear that the anti-Gm(n) was unable to form precipitates with Gm(n+) proteins without the assistance of anti-γ2 antibodies. Results obtained in radial immunodiffusion with serum samples of known Gm genotype showed that the antiserum differentiates three types of individuals: homozygous Gm(n− /n−), heterozygous Gm(n+ /n−) and homozygous Gm(n+ /n+).