Alterations in reactivity of the blood factors of cattle red cells after pronase treatment

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Abstract
Pronase treatment of cattle red cells produced various effects: (a) an increase in reactivity of the J factor and evolution of a specific cryptoantigen; (b) decrease in the A-B, G2, K, I1, O2, O3, P, Q, T1, Y2, A', B', E'2, E'3, I', K', O'-L'-V-L and M2 factors, but (c) no change in the specifity or in the titre of the remaining 16 different blood factors. Most of the pronase-affectable blood factors were destroyed in a rather narrow but characteristic range of pronase treatment intensities. However, at like intensities, variations were demonstrable due to the fact that the blood factor occurred (a) in red cells from different individuals, and (b) in different phenogroups or subgroups of the B locus.