PREPARATION AND CHARACTERIZATION OF MONOIODOINSULIN
- 1 May 1974
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 6 (3) , 157-163
- https://doi.org/10.1111/j.1399-3011.1974.tb02374.x
Abstract
Monoiodoinsulin has been prepared from heterogeneous iodoinsulin containing on an average 1 I/mole using ion exchange chromatography. The isolated monoiodoinsulin showed in contrast to the iodinated insulin essentially a single band examined by disc‐electrophoresis. The iodine content as judged by the specific radioactivity was 1.0 atom of iodine per molecule of insulin. The distribution of the iodine was 1% in the B‐chain and 99%, in the A‐chain, 30% in Tyr A14 and 70% in Tyr A19 respectively. The biological activity of the isolated monoiodoinsulin was 67% in comparison to crystalline insulin tested on isolated rat fat cells. The immunological reactivity using the double‐antibody method with pre‐precipitation was unchanged in comparison to crystalline insulinThis publication has 29 references indexed in Scilit:
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