Partial Purification and Characterization of Syrian Hamster Interferon

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Abstract
Syrian golden hamster interferon was made by stimulating secondary or benzo(a)pyrene-transformed embryo cells with Newcastle disease virus. Titers of 1000-6000 U/ml of tissue culture fluid were obtained. The ionic properties of this interferon were characterized by chromatography on cation and anion exchangers and also by isoelectric focusing when 2 components were observed: a major component, pI (isoelectric point) 5.8 and a minor component, pI 6.6. Hamster interferon was partially purified on a tandem of sorbents of diverse chromatographic bias: anion exchanger .fwdarw. metal chelate .fwdarw. hydrophobic ligand. The purified preparation had a specific activity of about 1 .times. 106 U/mg protein; the recovery of activity was nearly complete. The apparent MW of hamster interferon, as estimated by SDS(sodium dodecyl sulfate)-polyacrylamide gel electrophoresis under non-reducing conditions, is 17,000. Crude Syrian hamster interferon preparations had some activity (against vesicular stomatitis virus) on mouse cells; this heterologous activity was entirely due to a small subpopulation of interferon molecules which could be isolated on phenylagarose.

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