Dielectric spectroscopy of mammalian cells
- 1 January 1993
- journal article
- Published by Springer Nature in Cytotechnology
- Vol. 13 (3) , 195-202
- https://doi.org/10.1007/bf00749815
Abstract
Low-frequency dielectric spectroscopy has been usedin situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is characterized by a somewhat lower degree of resolution. Suggestions are made on how to determine the capacitance of the spent growth medium alone, still keeping the probe inserted in the bioreactor. This will make dielectric spectroscopy the first trulyin situ, on-line, in real time, non-invasive measure of the biomass.Keywords
This publication has 9 references indexed in Scilit:
- EVOLUTION OF THE CAPACITANCE OF CYTODEX 3 MICROBEADS SUSPENDED IN GROWTH MEDIUMPublished by Elsevier ,1994
- Dielectric spectroscopy of mammalian cellsCytotechnology, 1993
- On-line biomass monitoring by capacitance measurementJournal of Biotechnology, 1992
- The dielectric permittivity at radio frequencies and the bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell culturesJournal of Biotechnology, 1991
- Evidence from its temperature dependence that the ?-dielectric dispersion of cell suspensions is not due solely to the charging of a static membrane capacitanceEuropean Biophysics Journal, 1990
- Substitution and spreadsheet methods for analysing dielectric spectra of biological systemsEuropean Biophysics Journal, 1990
- Microbeads and anchorage-dependent eukaryotic cells: The beginning of a new era in biotechnologyPublished by Springer Nature ,1989
- Growth of animal cells on microbeads III: Semi continuous in situ estimation of numbersPublished by Elsevier ,1987
- Growth of animal cells on microbeads. I.In situ estimation of numbersBiotechnology & Bioengineering, 1986