Genes with internal repeats require the THO complex for transcription

26 September 2006

journal article
research article
Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences

Vol. 103 (39) , 14423-14428
https://doi.org/10.1073/pnas.0606546103

Abstract

The evolutionarily conserved multisubunit THO complex, which is recruited to actively transcribed genes, is required for the efficient expression of FLO11 and other yeast genes that have long internal tandem repeats. FLO11 transcription elongation in Tho⁻ mutants is hindered in the region of the tandem repeats, resulting in a loss of function. Moreover, the repeats become genetically unstable in Tho⁻ mutants. A FLO11 gene without the tandem repeats is transcribed equally well in Tho⁺ or Tho⁻ strains. The Tho⁻ defect in transcription is suppressed by overexpression of topoisomerase I, suggesting that the THO complex functions to rectify aberrant structures that arise during transcription.

Keywords

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