Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4
- 1 July 1990
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 4 (7) , 1199-1205
- https://doi.org/10.1111/j.1365-2958.1990.tb00695.x
Abstract
Transfer of donor cell proteins to the recipient bacterium was examined in F- and RP4-mediated conjugation. Transfer of a 120kD polypeptide, identified as the larger product of the plasmid DNA primase gene, was readily detected during RP4-promoted conjugation. The protein was transmitted to the cytoplasm of the recipient, presumably complexed to the transferred ssDNA. F DNA was transferred without detectable association with any cytoplasmic tra protein or with the ssDNA-binding protein encoded by the plasmid. However, a 92 kD protein, possibly F TraD product, was transmitted to the membrane fraction of the recipient cell.This publication has 32 references indexed in Scilit:
- Cooperative interaction of Agrobacterium VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process.Proceedings of the National Academy of Sciences, 1989
- Evidence that DNA helicase I and oriT site-specific nicking are both functions of the F TraI proteinJournal of Molecular Biology, 1988
- Role of IncP-1 plasmid primase in conjugation betweenPseudomonasspeciesFEMS Microbiology Letters, 1988
- Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA.Proceedings of the National Academy of Sciences, 1987
- Organization and regulation of the conjugation genes of IncI1 plasmid ColIb-P9Plasmid, 1987
- THE CONJUGATION SYSTEM OF F, THE FERTILITY FACTOR OF ESCHERICHIA COLIAnnual Review of Genetics, 1986
- Plasmid RP4 encodes two forms of a DNA primaseMolecular Genetics and Genomics, 1984
- Release of a special fraction of the outer membrane from both growing and phage T4-infected Escherichia coli BBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- The transposon Tn1 as a probe for studying ColE1 structure and functionMolecular Genetics and Genomics, 1977
- Limits on material transfer during F+ × F− matings in Escherichia coli K12Journal of Molecular Biology, 1965