SOURCES OF VARIANCE IN MEASUREMENT OF INTRAPULMONARY KILLING OF BACTERIA

  • 1 January 1976
    • journal article
    • research article
    • Vol. 87  (3) , 544-558
Abstract
Sources of variability are described for a method used for the quantitative measurement of pulmonary resistance to inhaled bacteria in individual animals. Factors contributing significantly to variability include aerosol exposure chamber design, bacterial species used for aerosol challenge, location of animals in the chamber and individual intrapulmonary bacterial killing rates. Laboratory error accounted for a small portion of the variance. With appropriate modifications in protocol, statistical design and analysis experiments can be performed with increased accuracy, thereby reducing the number of animals needed for each experiment.