Sites of aluminum accumulation in bone marrow: study using electron microscopy, ionic microscopy and X‐ray microanalysis

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Abstract
Two methods of analytical microscopy have been used to study the distribution of aluminum in bone marrow of rats intoxicated by aluminum gluconate. Images of the distribution of aluminum in a field of 250 microns in diameter were obtained by analytical ion microscopy. They show that this element was concentrated in spots, associated with iron or alone, in the cytoplasm of some cells. Electron Probe Microanalysis (EPMA) has shown that aluminum concentration occurred in cells of the reticulo‐endothelial system, principally in the reticular cells of erythroblastic islets. In cells of the reticuloendothelial system, aluminum was observed in intracytoplasmic organelles having ultrastructural characteristics of lysosomes or phagolysosomes. In these organelles, aluminum is always associated with phosphorus and sometimes with iron. No cytoplasmic or nuclear aluminum accumulation was detected in any other variety of bone marrow cells. The consequences of the selective accumulation of aluminum in the cytoplasm of reticular cells of erythroblastic islets for the maturation of erythrocytes are discussed.