In-vitro Sensitization of Monkey Lung Fragments with Human Ragweed Atopic Serum

Publisher Website
Google Scholar
Abstract
A method is described which employs rhesus monkey lung tissue for in vitro detection of reagins in human atopic sera. Fragments of monkey lung were incubated with ragweed allergic sera of known P-K [Prausnitz-Kustner assay] and hemagglutination titers. After washing, the fragments were incubated with the solutions of various allergens and the smooth muscle stimulating activity of the supernatants were determined on guinea pig ileum preparation standardized with and expressed in histamine. Exposure to ragweed allergen of fragments incubated with ragweed allergic sera resulted in the release oPsmooth muscle stimulators. The reaction seems to be specific because heterologous allergens were without effect; the amount of smooth muscle stimulators found in the supernatants showed a good correlation with P-K but not with hemagglutination titers; incubation of lung fragments with ragweed allergic sera, previously heated for 1 hr. at 56[degree] C, did not result in release of smooth muscle stimulants but the hemagglutination titers of these sera remained unchanged.

This publication has 2 references indexed in Scilit: