The mechanism of Klebsiella pneumoniae nitrogenase action. Simulation of the dependences of H2-evolution rate on component-protein concentration and ratio and sodium dithionite concentration

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Abstract
The rate constants of Lowe and Thorneley stimulated the rate of H2 evolution, under various conditions, from nitrogenase isolated from K. pneumoniae. These rates depend on the ratio and concentrations of the MoFe protein and Fe protein that comprise nitrogenase. The simulations explain the shapes of protein titration and dilution effect curves. The concept of an apparent Km for the reductant Na2S2O4 is invalid, since the dependence of H2-evolution rate on the square root of S2O42-concentration is not hyperbolic and depends on the ratio and absolute concentrations of the MoFe protein and Fe protein.