Assay of trimethoprim, sulfamethoxazole and its N4‐acetyl metabolite in biological fluids by high‐pressure liquid chromatography

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Abstract
A reversed‐phase HPLC assay method was developed to determine levels of the drugs trimethoprim, sulfamethoxazole and the N4‐acetyl metabolite of the latter in human blood plasma and urine. The organic substances are extracted with ethyl acetate by a single extraction at pH 6.8. The method is rapid, accurate, sensitive and precise and well suited to routine determination of these drugs on patients during clinical pharmacokinetic investigations.